The Effects of Angiotensin II, Olmesartan and PD123,319 on Protein Kinase C in STZ Induced Rat Vascular Smooth Muscle Cell Culture

Protein kinase C (PKC) is a promoter enzyme that plays vital role in signal transduction of vascular smooth muscle cells (VSMCs). It has numerous functions, such as cell growth, cytokine activation and angiogenesis. But, these mechanisms are deteriorating diabetes mellitus. Angiotensin II (Ang II) effects on structure binding Ang type-1 type-2 receptors (ATR1 ATR2) stimulates PKC mostly through ATR1s. We aim to investigate the II, Olmesartan PD123,319 levels healthy streptozotocin (STZ) induced rat VSMCs (H-VSMCs STZ-VSMCs) this study. The primary culture were isolated from thoracic aorta STZ (45 mg/kg, given via tail vein) Wistar rats. measured H-VSMCs STZ-VSMCs by enzyme-linked immunosorbent assay (ELISA). In H-VSMCs, group compared control group, decreased significantly (P = 0.000); whereas, control, higher, but not 0.088). increased II+Olmesartan 0.000) II+PD123,319 groups STZ-VSMCs, 0.001) significantly. its seem modulate each other may have relationship hyperglycemic conditions. Also, considering that, ATR1 blocker (ATR1B) ATR2 (ATR2B) be protective against injury reducing STZ-VSMCs.